Đầu năm học 2018-2019, tháng 8-2018, niềm vui nhỏ đến với nhóm Giảng viên Khoa Sinh học, Trường Đại học Sư phạm Thái Nguyên, bài báo về biểu hiện gen CrDAT từ cây Dừa cạn đã được xuất bản trên Tạp chí quốc tế Scopus, Australian Journal of Crop Science.
Abstract: Catharanthus roseus (L.) G. Don contains about 130 types of alkaloids, including vincristine and vinblastine, which are outstanding drugs for cancer. The C. roseus deacetylvindoline-4-O-acetyl transferase (CrDAT) is a key enzyme which catalyzes the second to the final reactions in the vindoline way. The low content of indole alkaloid in C. roseus plants and the high cost of indole alkaloid production have promted many research to improve indole alkaloid yield in this plant. The aim of this work was to express recombinant CrDAT in tobacco, a model plant, to create the basis for the overexpression of the gene encoding CrDAT (GenBank LN809930) in C. Roseus plants. In this study, the 35S-DAT-cmyc structure was transferred to tobacco and the transgenic tobacco lines was generated. The T1 generation was then analyzed by Western blot method and ELISA analysis. Southern blot assays confirmed that the CrDAT gene was completely introduced into tobacco genome by Agrobacterium-mediated transformation. The recombinant CrDAT protein of 51.5 kDa in size was successfully expressed at the seven transgenic tobacco lines. The recombinant CrDAT protein content of transgenic tobacco lines were 2.75 – 5.35 (ug. mg-1 of total protein) range and the recombinant CrDAT protein content of the T0-1 line was highest (5.35 ug. mg-1 of total protein).
Keywords: Agrobacterium-mediated transformation,recombinant CrDAT protein, transgenic tobacco, viblastine, vincristine
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Xin mới đọc tại: https://www.cropj.com/bui_12_7_2018_1139_1143.pdf.
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